Expression of a single lignin peroxidase-encoding gene in Phanerochaete chrysosporium strain ME446.
Identifieur interne : 000E73 ( Main/Exploration ); précédent : 000E72; suivant : 000E74Expression of a single lignin peroxidase-encoding gene in Phanerochaete chrysosporium strain ME446.
Auteurs : C M James [Royaume-Uni] ; M S Felipe ; P F Sims ; P. BrodaSource :
- Gene [ 0378-1119 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN fongique (MeSH), ARN fongique (biosynthèse), Basidiomycota (enzymologie), Basidiomycota (génétique), Cartographie chromosomique (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Gènes fongiques (MeSH), Lignine (métabolisme), Mutation (MeSH), Peroxidases (génétique), Peroxidases (métabolisme), Polymorphisme de restriction (MeSH), Séquence nucléotidique (MeSH), Transcription génétique (MeSH).
- MESH :
- biosynthèse : ARN fongique.
- enzymologie : Basidiomycota.
- génétique : Basidiomycota, Peroxidases.
- métabolisme : Lignine, Peroxidases.
- ADN fongique, Cartographie chromosomique, Données de séquences moléculaires, Expression des gènes, Gènes fongiques, Mutation, Polymorphisme de restriction, Séquence nucléotidique, Transcription génétique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Basidiomycota (enzymology), Basidiomycota (genetics), Chromosome Mapping (MeSH), DNA, Fungal (MeSH), Gene Expression (MeSH), Genes, Fungal (MeSH), Lignin (metabolism), Molecular Sequence Data (MeSH), Mutation (MeSH), Peroxidases (genetics), Peroxidases (metabolism), Polymorphism, Restriction Fragment Length (MeSH), RNA, Fungal (biosynthesis), Transcription, Genetic (MeSH).
- MESH :
- chemical , biosynthesis : RNA, Fungal.
- chemical , genetics : Peroxidases.
- chemical , metabolism : Lignin, Peroxidases.
- chemical : DNA, Fungal.
- enzymology : Basidiomycota.
- genetics : Basidiomycota.
- Base Sequence, Chromosome Mapping, Gene Expression, Genes, Fungal, Molecular Sequence Data, Mutation, Polymorphism, Restriction Fragment Length, Transcription, Genetic.
Abstract
A previously described linked set of lignin peroxidase-encoding genes (Lpo) from Phanerochaete chrysosporium (P.c.) ME446 is not expressed under standard growth conditions for ligninolytic activity. However, a single unlinked Lpo gene, not previously described in P.c. strain ME446, is expressed. The transcription start points of this gene are mapped and the gene is assigned to a genetic linkage group by the use of restriction-site polymorphism segregation analysis. No transcripts from Lpo-related genes, including that normally expressed in ME446, could be detected within RNA extracted from three nonligninolytic mutant strains, but a hyper-ligninolytic strain showed an increased level of Lpo expression. This increase is due to expression of additional Lpo genes, rather than to an increased level of transcription from the normally expressed sequence.
DOI: 10.1016/0378-1119(92)90577-c
PubMed: 1351020
Affiliations:
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ME446 is not expressed under standard growth conditions for ligninolytic activity. However, a single unlinked Lpo gene, not previously described in P.c. strain ME446, is expressed. The transcription start points of this gene are mapped and the gene is assigned to a genetic linkage group by the use of restriction-site polymorphism segregation analysis. No transcripts from Lpo-related genes, including that normally expressed in ME446, could be detected within RNA extracted from three nonligninolytic mutant strains, but a hyper-ligninolytic strain showed an increased level of Lpo expression. This increase is due to expression of additional Lpo genes, rather than to an increased level of transcription from the normally expressed sequence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1351020</PMID><DateCompleted><Year>1992</Year><Month>07</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN><JournalIssue CitedMedium="Print"><Volume>114</Volume><Issue>2</Issue><PubDate><Year>1992</Year><Month>May</Month><Day>15</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Expression of a single lignin peroxidase-encoding gene in Phanerochaete chrysosporium strain ME446.</ArticleTitle><Pagination><MedlinePgn>217-22</MedlinePgn></Pagination><Abstract><AbstractText>A previously described linked set of lignin peroxidase-encoding genes (Lpo) from Phanerochaete chrysosporium (P.c.) ME446 is not expressed under standard growth conditions for ligninolytic activity. However, a single unlinked Lpo gene, not previously described in P.c. strain ME446, is expressed. The transcription start points of this gene are mapped and the gene is assigned to a genetic linkage group by the use of restriction-site polymorphism segregation analysis. No transcripts from Lpo-related genes, including that normally expressed in ME446, could be detected within RNA extracted from three nonligninolytic mutant strains, but a hyper-ligninolytic strain showed an increased level of Lpo expression. This increase is due to expression of additional Lpo genes, rather than to an increased level of transcription from the normally expressed sequence.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>James</LastName><ForeName>C M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Felipe</LastName><ForeName>M S</ForeName><Initials>MS</Initials></Author><Author ValidYN="Y"><LastName>Sims</LastName><ForeName>P F</ForeName><Initials>PF</Initials></Author><Author ValidYN="Y"><LastName>Broda</LastName><ForeName>P</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Gene</MedlineTA><NlmUniqueID>7706761</NlmUniqueID><ISSNLinking>0378-1119</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012331">RNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><GeneSymbolList><GeneSymbol>LIG5</GeneSymbol><GeneSymbol>Lpo</GeneSymbol></GeneSymbolList><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="Y">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="N">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012331" MajorTopicYN="N">RNA, Fungal</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>5</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>5</Month><Day>15</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>5</Month><Day>15</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1351020</ArticleId><ArticleId IdType="pii">0378-1119(92)90577-C</ArticleId><ArticleId IdType="doi">10.1016/0378-1119(92)90577-c</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Broda, P" sort="Broda, P" uniqKey="Broda P" first="P" last="Broda">P. Broda</name><name sortKey="Felipe, M S" sort="Felipe, M S" uniqKey="Felipe M" first="M S" last="Felipe">M S Felipe</name><name sortKey="Sims, P F" sort="Sims, P F" uniqKey="Sims P" first="P F" last="Sims">P F Sims</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="James, C M" sort="James, C M" uniqKey="James C" first="C M" last="James">C M James</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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